| 使用说明书
TCH-C251-人乳腺癌细胞(L-15空气培养)(MDA-MB-436)培养要点.pdf
MX157A0_TCH-C251_人乳腺癌细胞(MDA-MB-436).pdf
细胞名称:人乳腺癌细胞
细胞简称:MDA-MB-436
产品货号:TCH-C251
种属来源:人
组织来源:乳腺
疾病特征:乳腺腺癌
细胞形态:多形
生长特性:贴壁生长
培养体系:L-15+20% FBS+0.01mg/ml Insulin+1% P/S
配套培养基货号:TCH-G251
传代比例:1:2-1:3,每2-3天换液一次
传代周期:24-48 h
培养条件:气相:空气,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997- 3001, 1990. PubMed: 2334903
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收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。