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MX261A0_TCH-C236_人食管鳞癌细胞(KYSE-150).pdf
由一名49岁日本女性接受放疗后从上(宫颈)食道切除的分化不良食管鳞状细胞癌(肿瘤侵入相邻结构)建立;被描述为携带扩增的癌基因,c-erb-B(8x)和cyclin D1(4x)并在裸鼠中产生肿瘤。
细胞名称:人食管鳞癌细胞
细胞简称:KYSE-150
产品货号:TCH-C236
种属来源:人
组织来源:食管
疾病特征:食管鳞癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:RPMI-1640 +10% FBS+1% P/S
配套培养基货号:TCH-G236
传代比例:1:2-1:4,每2-3天换液一次
传代周期:24-48 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Kanda, Y., Nishiyama, Y., Shimada, Y., Imamura, M., Nomura, H., Hiai, H., Fukumoto, M. (1994). Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines. International journal of cancer.Journal international du cancer 58 ( 2 ): 291-297 . Http://www.ncbi.nlm.nih.gov/pubmed/7913084 Shimada, Y., Imamura, M., Wagata, T., Yamaguchi, N., Tobe, T. (1992). Characterization of 21 newly established esophageal cancer cell lines. Cancer 69 ( 2 ): 277-284 . Http://www.ncbi.nlm.nih.gov/pubmed/1728357 Shimada, Y., Imamura, M. (1993). Prognostic significance of cell culture in carcinoma of the oesophagus. The British journal of surgery 80 ( 5 ): 605-607 . Http://www.ncbi.nlm.nih.gov/pubmed/8518899 Tanaka, H., Shibagaki, I., Shimada, Y., Wagata, T., Imamura, M., Ishizaki, K. (1996). Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors. International journal of cancer.Journal international du cancer 65 ( 3 ): 372-376 . Http://www.ncbi.nlm.nih.gov/pubmed/8575860
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收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。