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MX264A0_TCH-C188_人子宫内膜腺癌细胞（HEC-1-A）.pdf

这株细胞及其亚株HEC-1-B是H. Kuramoto及其同事1968年从一位IA期子宫内膜癌患者身上分离得到的。

细胞名称：人子宫内膜腺癌细胞

细胞简称：HEC-1-A

产品货号：TCH-C188

种属来源：人

组织来源：子宫

疾病特征：子宫内膜腺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：McCOY's 5A＋10% FBS＋1% P/S

配套培养基货号：TCH-G188

传代比例：1:2-1:4，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Kuramoto H . Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779 Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Presta M , et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185 Maggi M , et al. Platelet-activating factor mediates an autocrine proliferative loop in the endometrial adenocarcinoma cell line HEC-1A. Cancer Res. 54: 4777-4784, 1994. PubMed: 7520361 Kuramoto H , et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779 Hendricks DT , et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed:9187105

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。