产品名称:3T3-L1细胞hFASN基因敲除株
产品简称:3T3-L1 KO-hFASN
产品货号:CGKO-M2261
修饰基因:hFASN
基因ID:14104
修饰类型:敲除
转导方法:蛋白电转
抗性基因:—
克隆类型:纯合子
细胞鉴定:PCR,测序
种属来源:小鼠
组织来源:胚胎
疾病特征:/
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养体系:DMEM-H+10%CS(新生牛血清)+1%P/S
配套培养基货号:TCM-G702
传代比例:1:3-1:4,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 4426090
Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 1977
Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
Scherer PE, et al. Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc. Natl. Acad. Sci. USA 93: 131-135, 1996. PubMed: 8552590
Kallen CB, Lazar MA. Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 93: 5793-5796, 1996. PubMed: 8650171
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