产品名称:Jurkat细胞ATM基因敲除株
产品简称:Jurkat KO-hATM
产品货号:CGKO-M2203
修饰基因:hATM
基因ID:472
修饰类型:敲除
转导方法:蛋白电转
抗性基因:—
克隆类型:纯合子
细胞鉴定:PCR,测序
种属来源:人
组织来源:外周血
疾病特征:急性T淋巴细胞白血病
细胞形态:淋巴母细胞样
生长特性:悬浮生长
培养体系:RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
配套培养基货号:TCH-G225
传代比例:1:3-1:6,每2-3天换液一次
传代周期:24-48 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
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