产品名称：NCI-H1299细胞hUBE3A基因敲除株

产品简称：NCI-H1299 KO-hUBE3A

产品货号：CGKO-M0007

修饰基因：hUBE3A

基因ID：7337

修饰类型：敲除

转导方法：蛋白电转

抗性基因：—

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：肺

疾病特征：非小细胞肺癌

细胞形态：上皮样/多角形

生长特性：贴壁生长

培养体系：RPMI-1640+10%FBS+1%P/S

配套培养基货号：TCH-G269

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350

This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.

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