产品名称：ACHN细胞hTNFSF9基因敲除株

产品简称：ACHN KO-hTNFSF9

产品货号：CGKO-M2294

修饰基因：hTNFSF9

基因ID：8744

修饰类型：敲除

转导方法：蛋白电转

抗性基因：—

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：肾

疾病特征：肾细胞腺癌

细胞形态：圆形

生长特性：悬浮生长

培养体系：DMEM-H+10% FBS+1%Glutamax+1% Sodium Pyruvate+1%NEAA+1%P/S

配套培养基货号：TCH-G122

传代比例：1:2-1:3，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900

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