产品名称：MDA-MB-468细胞hRALA基因敲除株

产品简称：MDA-MB-468 KO-hRALA

产品货号：CGKO-M2191

修饰基因：hRALA

基因ID：10818

修饰类型：敲除

转导方法：质粒电转

抗性基因：Puro

克隆类型：纯合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：乳腺

疾病特征：乳腺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：L-15+10%FBS+1%P/S

配套培养基货号：TCH-G253

传代比例：1:2-1:3，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：空气，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591

Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847

Tumors developed within 21 days at 100% frequency (5/5).

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。