产品名称：THP-1细胞SRSF2基因点突变细胞株

产品简称：THP-1 MU-SRSF2

产品货号：CGMU-M2131

修饰基因：SRSF2_p.P95H_c.284C>A_c.285C>T

基因ID：6427_NM_001195427.2

转导方法：蛋白电转

克隆类型：杂合子

细胞鉴定：PCR，测序

种属来源：人

组织来源：外周血

疾病特征：急性单核细胞白血病

细胞形态：单核细胞样

生长特性：悬浮生长

培养体系：RPMI-1640+10%FBS+0.05 mM β-mercaptoethanol+1%P/S

配套培养基货号：TCH-G361

传代比例：1:3-1:6，每2-3天换液一次

传代周期：24-48 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

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Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819

Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812

Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011

Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838

Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727"

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