产品名称:Huh7细胞hSlc39a1基因敲除株
产品简称:Huh7 KO-hSlc39a1
产品货号:CGKO-M2476
修饰基因:hSlc39a1
基因ID:27173
修饰类型:敲除
转导方法:蛋白法
抗性基因:—
克隆类型:纯合子
细胞鉴定:PCR,测序
种属来源:人
组织来源:肝脏
疾病特征:肝癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S
配套培养基货号:TCH-G217
传代比例:1:2-1:3,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)
Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)
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