产品说明书:
HyCyte®永生化SD大鼠骨髓间充质干细胞BMRS-C106I.pdf
细胞名称:HyCyte®永生化SD大鼠骨髓间充质干细胞
细胞简称:ISD BMSCs
产品货号:BMRS-C106I
种属来源:大鼠,Sprague Dawley品系
组织来源:骨髓
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:BMRS-G101
传代比例:1:2(发现有比较多的死细胞或培养基颜色较黄时,应换液。一般为每2-3天换液)
传代周期:48-72 h
传代能力:P20代以内
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存代次:P3
冻存条件:海星干细胞专研冻存液HyCyte® One Step Freezing Medium(Cat. SCCP-R201)
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
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Tondreau T,Lagneaux L,Dejeneffe M 、et al . Isolation of BM mesenchymal stem cells by plastic adhesion or negative selec-tion: phenotype , proliferation kinetics and differentiation po-tential[J]. Cytotherapy ,2004,6( 4):372-379.
Alborxi A,Mac KG,Lackin CA,et al . Endochondral and intramembranous fetal bone development ,osteoblastic cell prolif-cration and expression of alkaline phosphatasc,rn-t wist. andhistone H4[J门.Crani fac Genet Der Boil .1996,16(2);94-98.
Pittenger MF.Mackay AM ,Beck SC,et al . Multilineage potential of adult hurman mesenchymal stem cells[J]. Science ,1999,284(5411):143-147.