产品说明书:
HyCyte®永生化SD大鼠脂肪间充质干细胞 ADRS-C106I.pdf
细胞名称:HyCyte®永生化SD大鼠脂肪间充质干细胞
细胞简称:ISD ADSCs
产品货号:ADRS-C106I
种属来源:大鼠,Sprague Dawley品系
组织来源:脂肪
细胞形态:成纤维细胞样
生长特性:贴壁生长
培养基货号:ADRS-G101
传代比例:1:2(发现有比较多的死细胞或培养基颜色较黄时,应换液。一般为每2-3天换液)
传代周期:48-72 h
传代能力:P20代以内
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存代次:P3
冻存条件:海星干细胞专研冻存液HyCyte® One Step Freezing Medium(Cat. SCCP-R201)
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
质量检测:细菌、真菌、支原体检测均为阴性
毒素检测:<10 EU/mL
参考文献:
Gaustad KG,Boquest AC,Anderson BE, et al. Differentiation of human adipose tissue stem cells using extracts of rat cardiomyo-cytes[J]. Biochem Biophys Res Commun , 2004,314( 2):420-427.
Yang LY ,Liu XM ,Sun B,et al. Adipose tissue-derived stromal cells express neuronal phenotypes [ J]. Chin Med J ( Engl ) ,2004, 117(3 ) ;425-429.
Seo MJ,Suh SY,Bae YC, et al. Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo[ J].Biochem Biophys Res Commun ,2005,328( 1);258-264.
Hattori H,Masuoka K,Sato M , et al. Bone formation using human adipose tissue-derived stromal cells and a biodegradablescaffold[J]、JBiomed Mater Res B Appl Biomater,2006,76( 1 ) :230-239.
Song HY , Jeon ES,Jung JS, et al. Oncostatin M induces proliferation of human adipose tissue-derived mesenchymal stem cells[J]. Int J Biochem Cell Biol , 2005 ,37( 11 ):2357-2365.