|   使用说明书

该细胞株是YaffeD,SaxelO建立的小鼠成肌细胞系的亚株。该细胞分化较快，可形成能收缩的微管，产生特异的肌肉蛋白。在骨形态形成蛋白（BMP-2）的作用下，该细胞可由成肌细胞分化为成骨细胞。检测发现该细胞鼠痘病毒阴性。

GFP标记小鼠成肌细胞(C2C12-GFP) 成肌管诱导图

细胞名称：GFP标记小鼠成肌细胞

细胞简称：C2C12-GFP

产品货号：TCM-C720G

种属来源：小鼠

组织来源：肌肉

疾病特征：/

细胞形态：成肌细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+3.0ug/mL Puromycin+1%P/S

配套培养基货号：TCM-G720G

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

"Qing Y, et al. Inhibitory effects of iron on bone morphogenetic protein 2-induced osteoblastogenesis. J. Bone Miner. Res. 26(6): 1188-1196, 2011. PubMed: 21308772

Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336

Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936

Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064

Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324

Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846

Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524"

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。