产品名称:HGC-27细胞hPIK3R1基因过表达稳转株
产品货号:CGSC-M25331
基因名称:hPIK3R1
基因ID: 5295
转导方法:转座子
转录本:[NM_181523.3]/3xFLAG
抗性基因:puro
荧光基因:EGFP
克隆类型:Poolcells
种属来源:人
组织来源:胃
疾病特征:胃癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:RPMI-1640+10%FBS+1%P/S
配套培养基货号:TCH-G202
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
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A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)
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Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat. Commun. 5:3830-3830(2014)
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
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