产品名称：HCT116细胞hHLF基因过表达稳转株

产品货号：CGSC-M25781

基因名称：hHLF

基因ID： 3131

转导方法：慢病毒

转录本：NM_002126.5

抗性基因：puro

荧光基因：EGFP

克隆类型：Poolcells

种属来源：人

组织来源：结肠

疾病特征：结肠癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系： McCoy's 5A+10%FBS+1%P/S

配套培养基货号：TCH-G185

传代比例：1:3-1:8，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO₂，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

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Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

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收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。