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MX023A4_TCH-C213_人结肠癌细胞（HT-29）.pdf

该细胞是1964年由FoghJ用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的。近来，已建株的培养细胞用含血清的McCoy＇s5a培养基培养。该细胞系在裸鼠中成瘤，也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素；表达尿激酶受体，但没有检测到血浆酶原活性；不表达CD4，但细胞表面表达半乳糖神经酰胺（HIV的可能替代受体）。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在273位密码子处发发生G

细胞名称：人结肠癌细胞

细胞简称：HT-29

产品货号：TCH-C213

种属来源：人

组织来源：结直肠

疾病特征：结直肠癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：McCoy's 5A+10%FBS+1%P/S

配套培养基货号：TCH-G213

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

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Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Chen TR, et al. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet. Cytogenet. 27: 125-134, 1987. PubMed: 3472642

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832

Fantini J, et al. Human colon epithelial cells productively infected with human immunodeficiency virus show impaired differentiation and altered secretion. J. Virol. 66: 580-585, 1992. PubMed: 1727501

Butzow R, et al. A 60-kD protein mediates the binding of transforming growth factor-beta to cell surface and extracellular matrix proteoglycans. J. Cell Biol. 122: 721-727, 1993. PubMed: 8335695

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Hanski C, et al. Tumorigenicity, mucin production and AM-3 epitope expression in clones selected from the HT-29 colon carcinoma cell line. Int. J. Cancer 50: 924-929, 1992. PubMed: 1372882

Reiter LS, et al. The role of the urokinase receptor in extracellular matrix degradation by HT29 human colon carcinoma cells. Int. J. Cancer 53: 444-450, 1993. PubMed: 8381394

Barnett SW, et al. Characterization of human immunodeficiency virus type 1 strains recovered from the bowel of infected individuals. Virology 182: 802-809, 1991. PubMed: 2024498

Shabahang M, et al. 1,25-Dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differentiation and growth inhibition. Cancer Res. 53: 3712-3718, 1993. PubMed: 8393379"

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收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。