产品说明书：

MU123A4_BMMC-C106_C57BL6小鼠骨髓间充质干细胞电子版.pdf

细胞名称：HyCyte™ C57BL/6小鼠骨髓间充质干细胞

细胞简称：C57BL/6 MSC

产品货号：BMMC-C106

种属来源：小鼠，C57BL/6品系

组织来源：骨髓

细胞形态：成纤维细胞样

生长特性：贴壁生长

培养基货号：BMMC-G101

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

毒素检测：＜10 EU/mL

参考文献：

Phinney DG,Kopen G, Isaacson Rl,et al. Plastic adherent stromal cells fron the bone marrow of commonly used strains of in-bred ice : variations in yield,growth,and differention[J.JCellBiochen,1999,72(4):570.

Friedenstein AJ , Chailakhyan RK.Gerasimov UV. Bone mnarrow osteogenic sten cells : in Vitro eultivation and transplantation indiffusion chambers[J]. Cell Tissue Kinet,1987,20(3);263.

Encina NR, Billotte WG,Hofmann MC. Immunomagnetic isolation of osteoprogenitors from human bone marrow stroma[J].Lab Invest,1999,79(4):449.

Zohar R, Sodek J,McCulloch CA. Characterization of stronal progenitor cells enriched by flow cytometry[J].Blood, 1997,90(9):3471.

Kicic A,Hall CM, Shen wY,et al.Are stem cell characteristics altered by disease state[JStem Cells Dev.2005,14(1):15.