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MX034A5_TCH-C258_人肝癌细胞（MHCC97H）.pdf

细胞名称：人肝癌细胞

细胞简称：MHCC97H

产品货号：TCH-C258

种属来源：人

组织来源：肝

疾病特征：高转移潜能肝癌细胞

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

配套培养基货号：TCH-G258

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

1. Dang H., Steinway S.N., Ding W., Rountree C.B.

Induction of tumor initiation is dependent on CD44s in c-Met(+) hepatocellular carcinoma.

BMC Cancer 15:161-161(2015)

2. Tian M.-M., Cheng H., Wang Z.-Q., Su N., Liu Z.-X., Sun C.-Q., Zhen B., Hong X.-C., Xue Y., Xu P.

Phosphoproteomic analysis of the highly-metastatic hepatocellular carcinoma cell line, MHCC97-H.

Int. J. Mol. Sci. 16:4209-4225(2015)

3. Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.

Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.

Hepatology 58:706-717(2013)

4. Li Y., Tian B., Yang J., Zhao L., Wu X., Ye S.-L., Liu Y.-K., Tang Z.-Y.

Stepwise metastatic human hepatocellular carcinoma cell model system with multiple metastatic potentials established through consecutive in vivo selection and studies on metastatic characteristics.

J. Cancer Res. Clin. Oncol. 130:460-468(2004)

5. Ding S.-J., Li Y., Shao X.-X., Zhou H., Zeng R., Tang Z.-Y., Xia Q.-C.

Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials.

Proteomics 4:982-994(2004)

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