| 使用说明书
MX035A6_TCH-C259_人胰腺癌细胞(MIA PaCa-2).pdf
该细胞表达人集落刺激因子CFS-I和血浆酶原激活剂。
细胞名称:人胰腺癌细胞
细胞简称:MIA PaCa-2
产品货号:TCH-C259
种属来源:人
组织来源:胰腺
疾病特征:胰腺癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:DMEM-H+10%FBS+2.5%马血清+1% Sodium Pyruvate+1%P/S
配套培养基货号:TCH-G259
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918
收货当天如何处理细胞?
收到细胞后请务必仔细阅读产品资料,了解细胞相关信息,如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。