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MX041A3_TCH-C315_人成骨肉瘤细胞（Saos-2）.pdf

该细胞是FoghJ和TrempeG分离和鉴定的众多人类肿瘤细胞系中的一种；该细胞来自一位11岁的白人女性的骨肉瘤组织。患者经过放疗以及氨甲喋呤、阿霉素、长春新碱、环磷酰胺和aramycin-C等多种药物治疗。该细胞在免疫抑制小鼠中不致瘤，细胞表达表皮生长因子EGF受体、转化生长因子β（1型和2型）受体。 

细胞名称：人成骨肉瘤细胞

细胞简称：Saos-2

产品货号：TCH-C315

种属来源：人

组织来源：骨

疾病特征：骨肉瘤

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：McCOY's 5A+15%FBS+1%P/S

配套培养基货号：TCH-G315

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Banerjee C, et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. PubMed: 8643513

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Takeuchi Y, et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639

Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. PubMed: 7860643

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

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收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。