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MX048A3_TCH-C368_人组织细胞淋巴瘤细胞(U-937).pdf
该细胞是由NilssonK实验室于1974年从一名37岁的患有恶性组织细胞性淋巴瘤的白人男性的胸水中分离建立的。1979年来的研究显示该细胞在人混合淋巴细胞培养物上清、佛波酯、VitD3、γ-IFN、TNF和维A酸的诱导下可以向终末单核细胞分化。该细胞不合成免疫球蛋白,EBV阴性;可产生溶菌酶、β-2-微球蛋白,受PMA刺激后可产生TNF-α;表达C3R;可作转染宿主;表达Fas,对TNF和抗Fas的抗体敏感。
细胞名称:人组织细胞淋巴瘤细胞
细胞简称:U-937
产品货号:TCH-C368
种属来源:人
组织来源:胸膜/胸腔积液
疾病特征:淋巴瘤细胞
细胞形态:单核细胞
生长特性:悬浮生长
培养体系:RPMI-1640+10%FBS+1%P/S
配套培养基货号:TCH-G368
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890
. Gene expression during normal and malignant differentiation. London: Academic Press; 1985.
. International symposium on new trends in human immunology and cancer immunotherapy. Paris: Doin Editeurs; 1980.
Koren HS, et al. In vitro activation of a human macrophage-like cell line. Nature 279: 328-331, 1979. PubMed: 450085
Gidlund M, et al. Natural killer cells kill tumour cells at a given stage of differentiation. Nature 292: 848-850, 1981. PubMed: 7266653
Olsson I, et al. Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1 alpha,25-dihydroxycholecalciferol. Cancer Res. 43: 5862-5867, 1983. PubMed: 6315218
Morimoto H, et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. PubMed: 7684321
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