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MX052A3_TCH-C297_人前列腺癌细胞（PC-3）.pdf

PC-3源于一位62岁白人男性IV级前列腺腺癌患者的骨转移灶；有低水平的酸性磷酸酶活性和5-α-睾酮还原酶活性。

细胞名称：人前列腺癌细胞

细胞简称：PC-3

产品货号：TCH-C297

种属来源：人

组织来源：前列腺

疾病特征：前列腺癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：F12K+10%FBS+1%P/S

配套培养基货号：TCH-G297

传代比例：1:3-1:6，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482

Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522

Ohnuki Y, et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073

Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194

Umekita Y, et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218

Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。