| 使用说明书
MX065A5_TCO-C037_中国仓鼠卵巢癌细胞(CHO-S).pdf
细胞名称:中国仓鼠卵巢癌细胞
细胞简称:CHO-S
产品货号:TCO-C037
种属来源:仓鼠
组织来源:卵巢
疾病特征:卵巢癌
细胞形态:上皮细胞样
生长特性:悬浮生长
培养体系:CHO-S专用培养基+1%Glutamax
配套培养基货号:TCO-G037
传代比例:1:3-1:6,每2-3天换液一次
传代周期:48-72 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存 (推荐海星一步冻存液GUCP-R201)
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Puck TT, et al. Genetics of somatic mammalian cells III. Long-term cultivation of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed: 13598821
Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed: 14294058
Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736
Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403
Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351
Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752
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