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MX068A5_TCM-C724_小鼠T淋巴细胞（CTLL-2）.pdf

该细胞是源自C57BL/6的细胞毒性的T淋巴细胞。其生长依赖IL-2。 

细胞名称：小鼠T淋巴细胞

细胞简称：CTLL-2

产品货号：TCM-C724

种属来源：小鼠

组织来源：淋巴细胞

疾病特征：/

细胞形态：淋巴细胞样

生长特性：悬浮生长

培养体系：RPMI 1640 + 1%Glutamax +100 U/ml mouse Recombinant IL-2 + 10% FBS+1%P/S

配套培养基货号：TCM-G724

传代比例：1:2-1:3，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Gillis S, Smith KA. Long term culture of tumour-specific cytotoxic T cells. Nature 268: 154-156, 1977. PubMed: 145543

Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

Mazzaccaro RJ, et al. Major histocompatibility class I presentation of soluble antigen facilitated by Mycobacterium tuberculosis infection. Proc. Natl. Acad. Sci. USA 93: 11786-11791, 1996. PubMed: 8876215

Belani R, Weiner GJ. Expression of both B7-1 and CD28 contributes to the IL-2 responsiveness of CTLL-2 cells. Immunology 87: 271-274, 1996. PubMed: 8698390

Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

收货当天如何处理细胞？

收到细胞后请务必仔细阅读产品资料，了解细胞相关信息，如贴壁特性 (贴壁/悬浮/半贴壁半悬浮)、细胞形态、培养体系、传代比例和换液频率等。