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MX072A3_TCH-C269_人非小细胞肺癌细胞（NCI-H1299）.pdf

这株细胞来源于一个淋巴结转移。患者接受了初期放疗。细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。细胞可以合成0.1pmol/毫克蛋白的NMB蛋白，而不合成促胃液释放肽(GRP)。 

细胞名称：人非小细胞肺癌细胞

细胞简称：NCI-H1299

产品货号：TCH-C269

种属来源：人

组织来源：肺

疾病特征：非小细胞肺癌

细胞形态：上皮样/多角形

生长特性：贴壁生长

培养体系：RPMI-1640+10%FBS+1%P/S

配套培养基货号：TCH-G269

传代比例：1:3-1:6，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350

This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.

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