| 使用说明书
MX076A5_TCH-C238_人前列腺癌细胞(Lncap).pdf
LNCaP克隆FGC是由JS Horoszewicz等人于1977年从一名50岁的白人男性(血型B +)的左锁骨上淋巴结的穿刺活检中分离出来的,该确诊为转移性前列腺癌。
细胞名称:人前列腺癌细胞
细胞简称:Lncap
产品货号:TCH-C238
种属来源:人
组织来源:前列腺
疾病特征:前列腺癌
细胞形态:上皮细胞样
生长特性:贴壁生长
培养体系:RPMI-1640+10%FBS+1%P/S
配套培养基货号:TCH-G238
传代比例:1:2-1:3,每2-3天换液一次
传代周期:72-96 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Models for prostate cancer. 37New York: Liss; 1980.
Gibas Z, et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. PubMed: 6584201
Horoszewicz JS, et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. PubMed: 6831420
Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991
Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed: 8662737
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