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MX077A6_TCM-C787_小鼠胰岛β细胞株（min6）.pdf

细胞名称：小鼠胰岛β细胞株

细胞简称：min6

产品货号：TCM-C787

种属来源：小鼠

组织来源：胰岛

疾病特征：/

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DMEM-H+15%FBS+1%Glutamax+0.05 mM β-mercaptoethanol+1%P/S

配套培养基货号：TCM-G787

传代比例：1:2-1:3，每2-3天换液一次

传代周期：72-96 h

培养条件：气相：95%空气+5%CO²，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

1. Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.

A mass spectrometric-derived cell surface protein atlas.

PLoS ONE 10:E0121314-E0121314(2015)

2. Lee J.-S., Wu Y., Schnepp P., Fang J., Zhang X., Karnovsky A., Woods J., Stemmer P.M., Liu M., Zhang K., Chen X.

Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells.

Proteomics 15:1508-1511(2015)

3. Cheng K., Delghingaro-Augusto V., Nolan C.J., Turner N., Hallahan N., Andrikopoulos S., Gunton J.E.

High passage MIN6 cells have impaired insulin secretion with impaired glucose and lipid oxidation.

PLoS ONE 7:E40868-E40868(2012)

4. Grozinsky-Glasberg S., Shimon I., Rubinfeld H.

The role of cell lines in the study of neuroendocrine tumors.

Neuroendocrinology 96:173-187(2012)

5. Nakashima K., Kanda Y., Hirokawa Y., Kawasaki F., Matsuki M., Kaku K.

MIN6 is not a pure beta cell line but a mixed cell line with other pancreatic endocrine hormones.

Endocr. J. 56:45-53(2009)

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