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MX220A0_TCH-C161_人结肠癌细胞(COLO 205).pdf
该细胞系1957年由T.U.Sample等从患有结肠癌的70岁男性白人的腹水分离。 该病人在取腹水样品前已用5-氟尿嘧啶治疗4-6周。 角蛋白免疫过氧化物酶染色阳性。
细胞名称:人结肠癌细胞
细胞简称:COLO 205
产品货号:TCH-C161
种属来源:人
组织来源:结肠
疾病特征:结直肠腺癌
细胞形态:上皮细胞样
生长特性:半贴壁生长
培养体系:RPMI-1640+10% FBS+1% P/S
配套培养基货号:TCH-G161
传代比例:5×10^5-1×10^6cells/mL,每2-3天换液一次
传代周期:24-48 h
培养条件:气相:95%空气+5%CO2,温度:37℃
冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存
质量检测:细菌、真菌、支原体检测均为阴性
参考文献:
Semple TU , et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251 Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Bjork P , et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed: 7693697
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